Journal: Molecular cell
Article Title: Tma64 (eIF2D), Tma20 (MCT-1), and Tma22 (DENR) recycle post-termination 40S subunits in vivo
doi: 10.1016/j.molcel.2018.07.028
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Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Monoclonal Antibody c-Myc (9E10) Roche 1167203001 Rabbit Polyclonal Antibody GCD6 Covance (CM002) ( Bushman et al., 1993 ) Rabbit Polyclonal Anti-Histone H3 Antibody Abcam Ab1791 Bacterial and Virus Strains DH5alpha NEB C2987H Rosetta pLacI-RARE Novagen 71404 Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Cycloheximide Sigma C7698 RNase I Ambion AM2294 PNK NEB M0201L Universal miRNA cloning linker NEB S1315S T4 RNA ligase 2, truncated NEB M0242L Superscript III Reverse Transcriptase Invitrogen 18080044 Lyticase Sigma L2524 Creatine kinase Sigma 000000010127566001 Creatine phosphate Sigma 10621714001 RiboLock RNase Inhibitor Thermo Fisher Scientific EO0381 D-luciferin (Beetle Luciferin, Potassium Salt) Promega E1602 DNase I recombinant, RNase-free Roche 04-716-728-001 SuperScript III First-Strand Synthesis SuperMix for qRT-PCR Invitrogen 11752-050 Critical Commercial Assays QuikChange Lightning Site-Directed Mutagenesis Kit Agilent Technologies 210518 CircLigase ssDNA Ligase Kit Epicentre CL4115K Agilent High Sensitivity DNA Kit Agilent 5067-4626 RiboMAX Large Scale RNA Production Systems Promega P1300 Vaccinia Capping System NEB M2080S Brilliant III Ultra-Fast SYBR Green QPCR Master Mix Agilent Technologies 600882 Deposited Data Raw and analyzed data This paper GEO: {"type":"entrez-geo","attrs":{"text":"GSE108942","term_id":"108942"}} GSE108942 Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains A full list of yeast strains used in this study is provided in Table S1 .
Techniques: Recombinant, Clone Assay, Mutagenesis, SYBR Green Assay, Software